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co expression with λphosphatase54  (Addgene inc)


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    Structured Review

    Addgene inc co expression with λphosphatase54
    Co Expression With λphosphatase54, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/co+expression+with+%CE%BBphosphatase54/Lambda+Phosphatase+(Plasmid+%2379748)/pm41735282-267-8-21
    Average 94 stars, based on 23 article reviews
    co expression with λphosphatase54 - by Bioz Stars, 2026-09
    94/100 stars

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    Produced:

    Article Title: The N-Myc MB0-MBI region interacts specifically and dynamically with the N-lobe of Aurora kinase A.
    Article Snippet: The 6x-His and solubility tag was cleaved with SENP2 during dialysis at 4 °C overnight and the protein was purified through incubation with Ni-NTA resin before the final polishing step by a HiLoad 16/600 Superdex 75 column (Cytiva) using GF buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 10% glycerol and 10mM β-mercaptoethanol). .. Unphosphorylated AurA122-403 with mutations C290A:C393A was produced by co-expression with λphosphatase54 (a gift from John Chodera, Nicholas Levinson and Markus Seeliger (Addgene plasmid # 79748; http://n2t.net/addgene: 79748; RRID: Addgene_79748) in BL21(DE3) cells. ..

    Plasmid Preparation:

    Article Title: The N-Myc MB0-MBI region interacts specifically and dynamically with the N-lobe of Aurora kinase A.
    Article Snippet: The 6x-His and solubility tag was cleaved with SENP2 during dialysis at 4 °C overnight and the protein was purified through incubation with Ni-NTA resin before the final polishing step by a HiLoad 16/600 Superdex 75 column (Cytiva) using GF buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 10% glycerol and 10mM β-mercaptoethanol). .. Unphosphorylated AurA122-403 with mutations C290A:C393A was produced by co-expression with λphosphatase54 (a gift from John Chodera, Nicholas Levinson and Markus Seeliger (Addgene plasmid # 79748; http://n2t.net/addgene: 79748; RRID: Addgene_79748) in BL21(DE3) cells. ..



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    94
    Addgene inc co expression with λphosphatase54
    Co Expression With λphosphatase54, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/co+expression+with+%CE%BBphosphatase54/Lambda+Phosphatase+(Plasmid+%2379748)/pm41735282-267-8-21
    Average 94 stars, based on 1 article reviews
    co expression with λphosphatase54 - by Bioz Stars, 2026-09
    94/100 stars
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